|
KMID : 0357119940160040347
|
|
Korean Journal of Immunology
1994 Volume.16 No. 4 p.347 ~ p.356
|
|
|
Isolation and Sequence Determination of Human Papillomavirus Type 18E6 Genes from Korean Women and Production of Recombinant 18E6 Genes from Korean Women and Production of Recombinant 18E6 Protein in E. coli
|
|
진승원
양은경/손우익/김현수/최인성/박순희
|
|
|
Abstract
|
|
|
Human papillomavirus type 18 (HPV 18) has recently been closely linked with human cervieal cancers. One of its early gene, E6 has been shown to code for a transforming protein. We analyzed the nucleotide sequences of threes HPV 18E6 oncogenes
isolated
from korean women. One of them havintg exactily same nucleotide sequences to the reported transforming HPV 18E6 oncogene was coned into pET-3a wector to construct a recombinant plasmid pET-18E6 to produce authentic size recombinant 18E6 protein
in
E.
coli. We also isolated TrpE-18E6 The expression level of recombinant 18E6 protein reached over 30% of total E. coli proteins but the protein was produced as inclusion bodies.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|